Journal: Cell & Bioscience

Article Title: Circular RNA expression profile in blood according to ischemic stroke etiology

doi: 10.1186/s13578-020-00394-3

Figure Lengend Snippet: Distribution by chromosomes ( a ) and chromosome’s region ( b ). Differentially expressed circRNA distribution in chromosomes and chromosome´s region (exonic, intronic, antisense, sense overlapping and intergenic) for each comparison

Article Snippet: To this end, we used Arraystar Human circRNA Array (8 × 15 K, Arraystar), which surveys up to 13,617 probes to identify human circRNAs, in 30 acute stroke patients with different etiologies by TOAST classification [ ].

Techniques:

Journal: Cell & Bioscience

Article Title: Circular RNA expression profile in blood according to ischemic stroke etiology

doi: 10.1186/s13578-020-00394-3

Figure Lengend Snippet: Deregulated circRNA chosen for qPCR validation

Article Snippet: To this end, we used Arraystar Human circRNA Array (8 × 15 K, Arraystar), which surveys up to 13,617 probes to identify human circRNAs, in 30 acute stroke patients with different etiologies by TOAST classification [ ].

Techniques:

Journal: Cell & Bioscience

Article Title: Circular RNA expression profile in blood according to ischemic stroke etiology

doi: 10.1186/s13578-020-00394-3

Figure Lengend Snippet: RT-qPCR validation of hsa_circRNA_102488. a Schematic representation of the UBA52 gene, origin of hsa_circRNA_102488. The graph shows the map of hsa_circRNA_102488 which is originated from exons 2 and 3 of its host mRNA (NCBI RefSeq track shown in the UCSC Genome Browser). b hsa_circRNA_102488 backsplicing. The Sanger-Sequencing electropherogram specifies the sequence of the backspliced junction that results in the circRNA formation. c – e hsa_circRNA_102488 and UBA52 mRNA expression levels between atherotrombotic vs cardioembolic samples. Percentage of hsa_circRNA_102488 expression relative to GAPDH mRNA ( c ) or UBA52 mRNA ( d ) is decreased in atherotrombotic with respect to cardioembolic stroke patients. However, we found similar expression levels of UBA52 mRNA in atherotrombotic and cardioembolic samples groups ( e ). Error bars: 95% CI. **p-value < 0.01; ***p-value < 0.001

Article Snippet: To this end, we used Arraystar Human circRNA Array (8 × 15 K, Arraystar), which surveys up to 13,617 probes to identify human circRNAs, in 30 acute stroke patients with different etiologies by TOAST classification [ ].

Techniques: Quantitative RT-PCR, Sequencing, Expressing

Journal: Cell & Bioscience

Article Title: Circular RNA expression profile in blood according to ischemic stroke etiology

doi: 10.1186/s13578-020-00394-3

Figure Lengend Snippet: GO analysis by mirPath v.3. The image shows the heatmaps corresponding to the analysis performed with the overrepresented miRNAs in the three comparisons (atherotrombotic-cardioembolic-undetermined) by TargetScan ( a ) and the analysis involving the target miRNAs for hsa_circRNA_102488 in accordance with molecular function ( b , by Tarbase) or biological process ( c , by microT-CDS)

Article Snippet: To this end, we used Arraystar Human circRNA Array (8 × 15 K, Arraystar), which surveys up to 13,617 probes to identify human circRNAs, in 30 acute stroke patients with different etiologies by TOAST classification [ ].

Techniques:

Journal: Cell & Bioscience

Article Title: Circular RNA expression profile in blood according to ischemic stroke etiology

doi: 10.1186/s13578-020-00394-3

Figure Lengend Snippet: hsa_circRNA_102488 impact pathways. The cartoon shows miRNAs (in blue) sharing a target site with hsa_circRNA_102488 (in black) and the pathways in which they converge (in dark orange), within those associated with the overrepresented miRNAs in the three comparisons (in light orange). RBPs sites matching to hsa_circRNA_102488 clustered around AGO2 (in dark green) and RBPs sites that matched to its flanking regions clustered around FUS (in light green). ARVC: Arrhythmogenic Right Ventricular Cardiomyopathy; HCM: Hypertrophic Cardiomyopathy

Article Snippet: To this end, we used Arraystar Human circRNA Array (8 × 15 K, Arraystar), which surveys up to 13,617 probes to identify human circRNAs, in 30 acute stroke patients with different etiologies by TOAST classification [ ].

Techniques:

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: The expression of circRNA SCARB1, mature miR-497 and miR-497 precursor was altered in HCC. HCC and paired non-tumor tissues were collected from the 64 HCC patients included in this study, and the expression of circRNA SCARB1 ( A ), mature miR-497 ( B ) and miR-497 precursor ( C ) in these tissue samples was determined by RT-qPCR. Average values of three technical replicates were used to express gene expression data in paired HCC and non-tumor tissues, ** p < 0.01.

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques: Expressing, Quantitative RT-PCR, Gene Expression

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: The Correlations Between Clinicopathological Variables and the Expression of circRNA SCARB1 in HCC

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques: Expressing, Infection

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: CircRNA SCARB1 and mature miR-497 were inversely correlated across HCC samples. Pearson’s correlation coefficient analysis was used to analyze the correlations between circRNA SCARB1 and mature miR-497 ( A ) or miR-497 precursor ( B ) across HCC tissue samples.

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques:

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: Overexpression of circRNA SCARB1 downregulated mature miR-497 in HCC cells. To explore the effects of overexpression of circRNA SCARB1 on the maturation of miR-497, SNU-423 and SNU-387 cells were transfected with either circRNA SCARB1 expression vector or miR-497 mimic, followed by the confirmation of transfections at 48 h post-transfection by RT-qPCR ( A ). The effects of overexpression of circRNA SCARB1 of the expression of mature miR-497 ( B ) and miR-497 precursor ( C ), as well as the effects of the transfection of miR-497 mimic on circRNA SCARB1 expression ( D ) were also analyzed by RT-qPCR. Mean ± SD values were used to express data of 3 biological replicates of in vitro cell experiments. * p < 0.05.

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques: Over Expression, Transfection, Expressing, Plasmid Preparation, Quantitative RT-PCR, In Vitro

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: Overexpression of circRNA SCARB1 increased HCC cell proliferation through miR-497. CCK-8 assay was performed to analyze the effects of overexpression of circRNA SCARB1 and miR-497 on the proliferation of SNU-423 ( A ) and SNU-387 cells ( B ). Mean ± SD values were used to express data of three biological replicates of in vitro cell experiments. * p < 0.05.

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques: Over Expression, CCK-8 Assay, In Vitro

Journal: Cancer Management and Research

Article Title: The Maturation of Tumor Suppressor miR-497 in Hepatocellular Carcinoma is Inhibited by Oncogenic circRNA SCARB1

doi: 10.2147/CMAR.S304125

Figure Lengend Snippet: Overexpression of circRNA SCARB1 increased HCC cell migration through miR-497. Transwell assay was conducted to analyze the effects of overexpression of circRNA SCARB1 and miR-497 on the migration of SNU-423 ( A and C ) and SNU-387 cells ( B and D ). Mean ± SD values were used to express data of three biological replicates of in vitro cell experiments. * p < 0.05.

Article Snippet: To overexpress circRNA SCARB1, pcDNA3.1(+) CircRNA Mini Vector (Addgene) was used as the backbone to construct the expression vector of circRNA SCARB1.

Techniques: Over Expression, Migration, Transwell Assay, In Vitro

Journal: Journal of Cellular and Molecular Medicine

Article Title: Circular RNA circANAPC2 mediates the impairment of endochondral ossification by miR‐874‐3p/SMAD3 signalling pathway in idiopathic short stature

doi: 10.1111/jcmm.16419

Figure Lengend Snippet: Microarray analysis of differentially expressed circRNAs. A, Four pairs of ISS patients and age‐/sex‐matched controls were screened via circRNA microarray analysis. Thirty differentially expressed circRNAs were shown, including top 22 up‐regulated circRNAs and top 8 down‐regulated circRNAs. B, 145 circRNAs were significantly differentially expressed, including 83 up‐regulation and 62 down‐regulation. C, D, The top 30 KEGG pathway and GO enrichment are exhibited. The ubiquitin‐mediated proteolysis and Wnt signalling pathway are closely associated with chondrocyte proliferation, hypertrophy and endochondral ossification. CircRNAs, circular RNAs; KEGG, Kyoto Encyclopedia of Genes and Genomes; GO, Gene ontology; and Wnt, wingless/integrated

Article Snippet: The labelled coding RNAs were then hybridized onto the Human circRNAs Array v2 (8 × 15 K, Arraystar).

Techniques: Microarray

Journal: Molecular Therapy. Nucleic Acids

Article Title: hsa_circNFXL1_009 modulates apoptosis, proliferation, migration, and potassium channel activation in pulmonary hypertension

doi: 10.1016/j.omtn.2020.09.029

Figure Lengend Snippet: circRNA profile, the genome-wide landscape view, and hierarchical clustering of PAH-COPD patients and healthy controls (A) Volcano plot shows the different expression of circRNAs in two groups of subjects. Red/green dots indicate circRNAs expressed more/less than a 1.5-fold change in PAH-COPD patients. (B) Numbers of dysregulated circRNAs coming from different chromosomes. (C) Pie chart represents the percentage of dysregulated circRNAs coming from different chromosome regions. (D) Heatmap of 158 most significantly dysregulated circRNAs. (E) Heatmap of the top 40 dysregulated circRNAs (20 upregulated and 20 downregulated). Green/red represent a low/high expression level. PAH-COPD, pulmonary arterial hypertension due to chronic obstructive pulmonary disease; circRNA, circular RNA. n = 3 pairs of subjects. Comparisons of data were acquired by a Student’s t test.

Article Snippet: The circRNAs were transcribed into fluorescent cRNA and hybridized onto the Arraystar human circRNA array (8 ×15K, Arraystar) using a random priming method.

Techniques: Genome Wide, Expressing

Journal: Cancer Management and Research

Article Title: Profiles of differentially expressed circRNAs in esophageal and breast cancer

doi: 10.2147/CMAR.S167863

Figure Lengend Snippet: General information of ESCC patients for microarray test

Article Snippet: The RNA was isolated with an RNeasy mini kit (Qiagen, Hilden, Germany) and analyzed using an 8*15K Arraystar human circRNA microarray V2 (Catalog No: AS-CR-H-V2.0).

Techniques: Microarray

Journal: Cancer Management and Research

Article Title: Profiles of differentially expressed circRNAs in esophageal and breast cancer

doi: 10.2147/CMAR.S167863

Figure Lengend Snippet: General information of BC patients for microarray test

Article Snippet: The RNA was isolated with an RNeasy mini kit (Qiagen, Hilden, Germany) and analyzed using an 8*15K Arraystar human circRNA microarray V2 (Catalog No: AS-CR-H-V2.0).

Techniques: Microarray